Dear all,

The setup seems to be quite clear.

Every semester we have at least five students coming into our lab to get involved in molecular biology techniques. Untill now, those who started their training with me, all had to go through the same basic steps. I will follow this method and we will write comprehensive protocolls about these steps.

The basic route in starting to work in our molecular biology lab was the following:

I. INTRODUCTORY PART

1. Introduction to the members of our lab.
2. Safety rules and regulations in the lab.
3. How to deal with the garbage, trash and other materials produced during the experiments.
4. How many tipes of water do we use?
5. What kind of reagents to we use?
6. Where and how do we store our reagents and our samples.

II. BASICS IN THE MOLECULAR BIOLOGY LAB

1. How can we protect our sample from degradation? If our sample is:
a. DNA
b. RNA
c Protein

2. DNA purification methods.
3. RNA purification methods.
4. Quallity control (QC) of DNA and RNA

III. BASIC TECHNIQUES TO GET USED TO LABWORK IN THE MOLECULAR BIOLOGY LAB

1. Working with plasimds:

a. Transformation
b. Growing of bacterial culture
c. Plasmid purification
d. Restriction analysis
e. Gel electrophoresis

2. Working in the cell culture lab:

a. Cell culturing basics
b. What is "sterile" in the issue culture environment?
c. Adherent and floating cells.
c. Making a passage.
d. Cell counting.

3. Transfection

a. Our methods used for transfection
b. QC
c. Sample preparation

4. PCR

a. Introduction into PCR
b. PCR and QPCR in practice
c. Analysis of the results

IV. ADVANCED TECHNIQUES IN THE MOLECULAR BIOLOGY LAB

We will elaborate this section later. We plan to include Western Blot, Protein purification, Modility Shift studies, Chromatin studies. etc.