The setup seems to be quite clear.
Every semester we have at least five students coming into our lab to get involved in molecular biology techniques. Until now, those who started their training with me, all had to go through the same basic steps. I will follow this method and we will write comprehensive protocols about these steps.
The basic route in starting to work in our molecular biology lab was the following:
I. INTRODUCTORY PART
1. Introduction to the members of our lab.
2. Safety rules and regulations in the lab.
3. How to deal with the garbage, trash and other materials produced during the experiments.
4. How many types of water do we use?
5. What kind of reagents to we use?
6. Liquid handling with pipettes.
7. Where and how do we store our reagents and our samples.
II. BASICS IN THE MOLECULAR BIOLOGY LAB
1. How can we protect our sample from degradation? If our sample is:
a. DNA
b. RNA
c Protein
2. DNA purification methods.
3. RNA purification methods.
4. Quality control (QC) of DNA and RNA
III. BASIC TECHNIQUES TO GET USED TO LABWORK IN THE MOLECULAR BIOLOGY LAB
1. Working with plasimds:
a. Transformation
b. Growing of bacterial culture
c. Plasmid purification
d. Restriction analysis (Restriction Enzymes)
e. Gel electrophoresis
2. Working in the cell culture lab:
a. Cell culturing basics
b. What is "sterile" in the issue culture environment?
c. Adherent and floating cells.
c. Making a passage.
d. Cell counting.
3. Transfection
a. Our methods used for transfection
b. QC
c. Sample preparation
4. PCR
a. Introduction into PCR
b. PCR and QPCR in practice
c. QPCR oligo design made easy
d. Analysis of the results
IV. ADVANCED TECHNIQUES IN THE MOLECULAR BIOLOGY LAB
We will elaborate this section later. We plan to include Western Blot, Protein purification, Mobility Shift studies, Chromatin studies. etc.
V. IGEM
a. iGEM intro
b. Prezi
VI. OTHER STUFF
a. Iterviews:
* Interview with Howard Wolinsky the US correspondent of EMBO Reports
b. Interesting stuff
* Hunting viruses with microarrays
* Bird flu solved Swine flu arrived
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